PHTaking a look at mrc-5 used in Vaccine
1. the immorality, the original sin, or would you like a product
of intentionally aborted fetus in your body?
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"Each of these cell lines started with cells
harvested from a deliberately aborted fetus
[177,178]. The cell lines are used to grow the
viruses, then discarded and not included in
vaccine formulations. These cell lines cannot form
a human being.
The WI-38 line was developed at the Wistar
Institute in Philadelphia in 1961, with lung cells
from a female fetus of 3 months gestation aborted
in Sweden, whose parents felt they had too many
children [175,176,179–183]. Similarly, British
scientists funded by the Medical Research Council
developed the MRC-5 line in September 1966 with
fetal lung fibroblasts “taken from a 14-week-old
male fetus removed for psychiatric reasons from a
27-year-old woman…” [179,184,185]. These cell
lines, still in use today, gradually replaced
primary cultures of monkey, duck, rabbit, chicken,
dog, or mouse tissue, an approach vulnerable to
contamination with viruses and bacteria
[175,176,183].
Vaccine manufacturers have few options for viral
culture media, for reasons of microbiology and
safety [175,176,179,186–190]. It is not possible
to simply replace one cell line with another,
because various viruses grow abundantly only in
some kinds of cell lines. WI-38 and MRC-5 lines
are well described and understood, with experience
accumulated via hundreds of millions of
vaccinations, important for safety-assessment
reasons.
The fetal origins of WI-38 and MRC-5 cell lines
pose an ethical or moral problem for people who
disapprove of abortion. Critically, the two
abortions were not conducted for the purpose of
harvesting the cells that were transformed into
these cell lines [177,178,191–194]. This lack of
intention is a key element in breaking the
complicity link that could otherwise make use of
the vaccines unacceptable. No additional abortions
are needed to sustain vaccine manufacture. "
** so mrc-5 is a product of a fetal abortion.
Though the Catholic Church has said its not
immoral anymore lol.
2. the effects
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Confocal immunofluorescent analysis
The 5× 105 cells were implanted onto a cell
culture dish for 24 hours (NEST Biotech, Hong
Kong, China) after culturing in MRC-5-CM for 14
days. Cells were fixed with paraformaldehyde for
30 minutes, then permeabilized with 0.1% Triton
X-100 for 10 minutes at room temperature, and
thereafter sealed with goat serum for 1 hour at
room temperature following primary antibodies
incubation in the dark for 24 hours at 4°C. Washed
three times with PBS, the cells were then
incubated with Alexa Flour® 488 IgG donkey anti-
mouse or anti-rabbit second antibodies (1:300,
Invitrogen, USA) in the dark for 1 hour at room
temperature. Fluorescence images were photographed
with confocal microscopy (Leica DMIRE2, Germany)
(at 10×63 magnification).
We found that the fibroblasts were activated by
the TGF-β and PDGF secreted by the tumor cells.
This resulted in fibroblasts showing enhanced
proliferation and IL-6 secretion. In turn, IL-6
secreted by activated fibroblasts boosted tumor-
cell proliferation and chemoresistance, but not
migration. Similar conclusions could be reached
using human TNBC cells and human fibroblasts.
** so mrc-5 fragments (IL-6) can cause cancer.
much debate in this nowadays. Now why do you need
these ingredients be injected into children?
Alternate studies of some scientists opposing shows
that there aren't really
viruses, but the processes/actions in culturing tissues
breakdown the cells (Dr. Lanka, Dr. Cowan and more),
and those fragments from thedecayed are mistakenly
called viruses. So the efficacy of vaccines are witch brew
and reallyhave not been scientifically proven as "not cure" or
preemptive of any disease, because injecting a
decayed matter into the body is just injecting a
decayed matter and has no logical effect of cure or immunity.
Lol. my pov from what researches say. Use logic.
1. the immorality, the original sin, or would you like a product
of intentionally aborted fetus in your body?
You do not have permission to view the full content of this post. Log in or register now.
"Each of these cell lines started with cells
harvested from a deliberately aborted fetus
[177,178]. The cell lines are used to grow the
viruses, then discarded and not included in
vaccine formulations. These cell lines cannot form
a human being.
The WI-38 line was developed at the Wistar
Institute in Philadelphia in 1961, with lung cells
from a female fetus of 3 months gestation aborted
in Sweden, whose parents felt they had too many
children [175,176,179–183]. Similarly, British
scientists funded by the Medical Research Council
developed the MRC-5 line in September 1966 with
fetal lung fibroblasts “taken from a 14-week-old
male fetus removed for psychiatric reasons from a
27-year-old woman…” [179,184,185]. These cell
lines, still in use today, gradually replaced
primary cultures of monkey, duck, rabbit, chicken,
dog, or mouse tissue, an approach vulnerable to
contamination with viruses and bacteria
[175,176,183].
Vaccine manufacturers have few options for viral
culture media, for reasons of microbiology and
safety [175,176,179,186–190]. It is not possible
to simply replace one cell line with another,
because various viruses grow abundantly only in
some kinds of cell lines. WI-38 and MRC-5 lines
are well described and understood, with experience
accumulated via hundreds of millions of
vaccinations, important for safety-assessment
reasons.
The fetal origins of WI-38 and MRC-5 cell lines
pose an ethical or moral problem for people who
disapprove of abortion. Critically, the two
abortions were not conducted for the purpose of
harvesting the cells that were transformed into
these cell lines [177,178,191–194]. This lack of
intention is a key element in breaking the
complicity link that could otherwise make use of
the vaccines unacceptable. No additional abortions
are needed to sustain vaccine manufacture. "
** so mrc-5 is a product of a fetal abortion.
Though the Catholic Church has said its not
immoral anymore lol.
2. the effects
You do not have permission to view the full content of this post. Log in or register now.
Confocal immunofluorescent analysis
The 5× 105 cells were implanted onto a cell
culture dish for 24 hours (NEST Biotech, Hong
Kong, China) after culturing in MRC-5-CM for 14
days. Cells were fixed with paraformaldehyde for
30 minutes, then permeabilized with 0.1% Triton
X-100 for 10 minutes at room temperature, and
thereafter sealed with goat serum for 1 hour at
room temperature following primary antibodies
incubation in the dark for 24 hours at 4°C. Washed
three times with PBS, the cells were then
incubated with Alexa Flour® 488 IgG donkey anti-
mouse or anti-rabbit second antibodies (1:300,
Invitrogen, USA) in the dark for 1 hour at room
temperature. Fluorescence images were photographed
with confocal microscopy (Leica DMIRE2, Germany)
(at 10×63 magnification).
We found that the fibroblasts were activated by
the TGF-β and PDGF secreted by the tumor cells.
This resulted in fibroblasts showing enhanced
proliferation and IL-6 secretion. In turn, IL-6
secreted by activated fibroblasts boosted tumor-
cell proliferation and chemoresistance, but not
migration. Similar conclusions could be reached
using human TNBC cells and human fibroblasts.
** so mrc-5 fragments (IL-6) can cause cancer.
much debate in this nowadays. Now why do you need
these ingredients be injected into children?
Alternate studies of some scientists opposing shows
that there aren't really
viruses, but the processes/actions in culturing tissues
breakdown the cells (Dr. Lanka, Dr. Cowan and more),
and those fragments from thedecayed are mistakenly
called viruses. So the efficacy of vaccines are witch brew
and reallyhave not been scientifically proven as "not cure" or
preemptive of any disease, because injecting a
decayed matter into the body is just injecting a
decayed matter and has no logical effect of cure or immunity.
Lol. my pov from what researches say. Use logic.