You are given the following DNA fragment to sequence:
5'-GCTTAGCATC-3'. You first clone the fragment in bacterial
cells to produce sufficient DNA for sequencing. You isolate the
DNA from the bacterial cells and carry out the dideoxy-sequencing
method. You then separate the products of the polymerization
reactions by gel electrophoresis. Draw the bands that should
appear on the gel from the four sequencing reactions.
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5'-GCTTAGCATC-3'. You first clone the fragment in bacterial
cells to produce sufficient DNA for sequencing. You isolate the
DNA from the bacterial cells and carry out the dideoxy-sequencing
method. You then separate the products of the polymerization
reactions by gel electrophoresis. Draw the bands that should
appear on the gel from the four sequencing reactions.
di